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Cyclosporine A에 의해 과증식된 조직의 치은섬유아세포에서 MMP-1,TIMP-1,TGF-β1,FGF-2,그리고 FGF-7의 mRNA 발현에 관한 연구
The mRNA expression of MMP-1,TIMP-1,TGF-β1,FGF-2 and FGF-7 in gingival fibroblasts induced by cyclosporine A

대한치과이식학회지 2008년 27권 1호 p.1 ~ 13

마평수, 황경균, 심광섭, 박창주, 이재선,

소속 상세정보

마평수 ( Ma Pyung-Soo ) - 한양대학교 의과대학 치과학교실 구강악안면외과
황경균 ( Hwang Kyung-Gyun ) - 한양대학교 의과대학 치과학교실 구강악안면외과
심광섭 ( Shim Kwang-Sub ) - 한양대학교 의과대학 치과학교실 구강악안면외과
박창주 ( Park Chang-Joo ) - 한양대학교 의과대학 치과학교실 구강악안면외과
이재선 ( Lee Jae-Sun ) - 한양대학교 의과대학 의생명연구소

KMID : 0362820080270010001

Abstract

Purpose: Cyclosporine A (CsA) is a widely used immunosuppresive agent with development of gingival overgrowth as a side effect. The mechanisms underlying this immunosuppressive activity are not fully understood. The progression of gingival overgrowth results from the accumulation of extracellular matrix. The purpose of this study was to investigate the mRNA expression of several growth factors associated with CsA according to the induction time and dose.

Materials & Methods: Gingival fibroblasts were obtained from gingival tissues of healthy donor and the patients treated with CsA. The cultured gingival fibroblasts were incubated with increasing concentration(0, 250, 500, 750, 1000, 1500, 2000 ng/ml) of CsA for 24, 48, and 72 hours, and the expression of MMP-1, TIMP-1, TGF-β1, FGF-2 and FGF-7 was determined by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results: The expression of MMP-1 was slightly decreased according to the concentration of treated CsA in 24 and 48 hours stimulation. There was no change in TIMP-1 expression. TGF-β1 showed the increased expression in 72 hours stimulation and FGF-2 expression was increased in 48 and 72 hours stimulation. The FGF-7 expression was significantly decreased in 48 and 72 hours stimulation.

Conclusions: We concluded that the gingival overgrowth might be related with TGF-β1, MMP-1 and FGF-2 expression associated with collagen metabolism. And the FGF-7 associated with keratinocyte growth was significantly related with CsA gingival overgrowth. We also speculated that there was no cellular change associated with CsA induction in gingival fibroblast.